Barnali Chaudhuri
Barnali Chaudhuri
Associate Professor
Faculty of Life Sciences and Biotechnology (FLSB)
Akbar Bhawan, Chanakyapuri
New Delhi – 110021

Profile

Professional experience: Research Scientist/Assistant Professor dual position at the Hauptman Woodward Institute and the Department of Structural Biology, SUNY, Buffalo (2006-2011).Post-doctoral research associate at the University of California, Los Angeles (2001-2006).Post-doctoral research associate at the Purdue University, Indiana (1999-2001).Understanding bacterial mitosisOur goal is to understand how segrosome, the mitotic-like chromosome trafficking device, segregates newly replicated chromosomes in the pre-divisional bacterial cell. Bacterial chromosome segregation cassette or the segrosome (ParABS) is composed of two proteins (ParA and ParB) and a set of centromere-like DNA sequences typically found near the origin of replication (parS). ParBs self-associate on the parS-proximal chromosomal DNA to form the partition assembly, a large nucleoprotein filament of unknown nature spanning several kilobases. The partition assembly serves to recruit ParA (an ATPase that probably forms a spindle-like filament to drive segregation) as well as SMC (Structural Maintenance of Chromosome that condenses DNA) proteins in several bacteria for DNA segregation. The mechanism of bacterial segrosome-mediated DNA movement is not understood.We are using a hybrid approach to elucidate the architectures of the segrosome assembly. Spatial organizations of these filaments will be elucidated using experiment-derived information on shape, size, periodicity, internal topology, interface and exposed surfaces - like solving a puzzle. In addition, we will find out how these filaments interact with each other, leading to DNA segregation. We are using a number of techniques including solution scattering with contrast variation, transmission electron microscopy, and crystallography.

Qualifications

  • Ph.D. from Uppsala University, Sweden , in 1998
  • M.Sc. from Indian Institute of Technology (IIT), Kharagpur , in 1991
  • B.Sc. from Presidency College, Calcutta University , in 1988

Recent Publications

  • Qian, S, Urban, V, Dean, R and Chaudhuri BN (*), "The internal organization of mycobacterial partition assembly: does the DNA wrap a protein core?" (PLOS ONE, in press) *- corresponding author
  • Chaudhuri, B. N. (*), Gupta, S., Urban, V. S., Chance, M., D'Mello, R., Smith, L., Lyons, K. & Gee, J. (2011). A combined global and local approach to elucidate spatial organization of the mycobacterial ParB-parS partition assembly. Biochemistry 50 (11), 1799-807.*- corresponding author
  • Chaudhuri, B. N. (*) and Dean, R. (2011) The evidence of large-scale DNA-induced compaction in the mycobacterial chromosomal ParB. Journal of Molecular Biology 413 (5), 2011, 901-907.*- corresponding author

Research Interests

  • Our principal research interest is to investigate how biological macromolecules function as molecular machines by virtue of their structural design.

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